ctfZeroPoint

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ctfZeroPoint is Eos's Command.


List of option

Main option

Option Essential/Optional Description Default
-o Optional Output: ASCII stdout
-RMax Optional max Spatial frequency[/A] 0.1
-dR Optional delta Spatial frequency[/A] 0.0001
-kV Optional Acceleration voltage[kV] 200
-Cs Optional Spherical aberration coefficient[mm] 2.1
-df Optional defocus: [A]:under(+) 27000
-dfMin Optional min defocus: [A] 0
-dfMax Optional max defocus: [A] 200000
-deltadf Optional delta defocus[A] 2000
-A Optional AmplitudeContrast/PhaseContrast 0.05
-N Optional Ordinal number of zero point 1.0
-Ain Optional Illumination Aperture[mrad] 0.02
-ctfMode Optional CTFMode 0
-c Optional ConfigurationFile NULL
-m Optional Mode 0
-h Optional Help  

-m details

Value Description
0 One zero point
1 All zero points
2 All zero points along defocus

CTFMode details

Value Description
0 Phase CTF
1 PhaseCTF + ratio*AmpCTF


Execution example

Case: Options only essential

[ZeroPointResult] AmpContrast        0.050000 
Amount of Defocus [A]  Number          R [A] ......... 
   27000.000000 1        0.038443 


Option -kV

Case: kV=100

[ZeroPointResult] AmpContrast        0.050000 
Amount of Defocus [A]  Number          R [A] ......... 
   27000.000000 1        0.031647 


Option -Cs

Case: Cs=5.0

[ZeroPointResult] AmpContrast        0.050000 
Amount of Defocus [A]  Number          R [A] ......... 
   27000.000000 1        0.038453 


Option -df

Case: df=20000

[ZeroPointResult] AmpContrast        0.050000 
Amount of Defocus [A]  Number          R [A] ......... 
   20000.000000 1        0.044674 


Option -A

Case: A=0.1

[ZeroPointResult] AmpContrast        0.100000 
Amount of Defocus [A]  Number          R [A] ......... 
   27000.000000 1        0.038443 


Option -N

Case: N=2

[ZeroPointResult] AmpContrast        0.050000 
Amount of Defocus [A]  Number          R [A] ......... 
   27000.000000 2        0.054377 


Option -ctfMode

Case: ctfMode=1

[ZeroPointResult] AmpContrast        0.050000 
Amount of Defocus [A]  Number          R [A] ......... 
   27000.000000 1        0.038136 


Option -m

Case: m=1, N=2

[ZeroPointResult] AmpContrast        0.050000 
Amount of Defocus [A]  Number          R [A] ......... 
   27000.000000 2       0.038443       0.054377


Case: m=2

Case: No other settings
[ZeroPointResult] AmpContrast        0.050000 
Amount of Defocus [A]  Number          R [A] ......... 
       0.000000               1       -1.000000
    2000.000000               1       -1.000000
    4000.000000               1       -1.000000
    6000.000000               1        0.081838
    8000.000000               1        0.070758
   10000.000000               1        0.063241
   12000.000000               1        0.057707
   14000.000000               1        0.053414
   16000.000000               1        0.049956

...

   80000.000000               1        0.022330
   82000.000000               1        0.022056
   84000.000000               1        0.021792
   86000.000000               1        0.021537
   88000.000000               1        0.021291
   90000.000000               1        0.021053
   92000.000000               1        0.020823
   94000.000000               1        0.020600
   96000.000000               1        0.020384
   98000.000000               1        0.020175
  100000.000000               1        0.019972

...

  190000.000000               1        0.014489
  192000.000000               1        0.014414
  194000.000000               1        0.014339
  196000.000000               1        0.014266
  198000.000000               1        0.014194
  200000.000000               1        0.014122


Case: dfMin=8000, dfMax=100000, deltadf=4000
[ZeroPointResult] AmpContrast        0.050000 
Amount of Defocus [A]  Number          R [A] ......... 
    8000.000000               1        0.070758
   12000.000000               1        0.057707
   16000.000000               1        0.049956
   20000.000000               1        0.044674
   24000.000000               1        0.040777
   28000.000000               1        0.037750
   32000.000000               1        0.035311
   36000.000000               1        0.033290
   40000.000000               1        0.031581
   44000.000000               1        0.030111
   48000.000000               1        0.028829
   52000.000000               1        0.027698
   56000.000000               1        0.026690
   60000.000000               1        0.025785
   64000.000000               1        0.024966
   68000.000000               1        0.024220
   72000.000000               1        0.023538
   76000.000000               1        0.022910
   80000.000000               1        0.022330
   84000.000000               1        0.021792
   88000.000000               1        0.021291
   92000.000000               1        0.020823
   96000.000000               1        0.020384
  100000.000000               1        0.019972