mrcImageNormalizing

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mrcImageNormalizing is Eos's Command that performs normalizing the value of image.


List of option

Main option

Option Essential/Optional Description Default
-i Essential Input: mrcImage NULL
-o Essential Output: mrcImage NULL
-A Optional A 1.0
-B Optional B 0.0
-ContourMin Optional ContourMin 0.0
-ContourMax Optional ContourMax 1.0
-ContourSolvent Optional ContourSolvent 0.0
-c Optional ConfigurationFile NULL
-m Optional Mode 0
-h Optional Help  

-m details

Value Description
0 Double Exponential: Solvent and Object

Fitting histgram to double exponentials as Solvent and Object

data = A*(data-MeanOfSolvent)/(MeanOfObject-MeanOfSolvent) + B
1 Min-Max: Background and Object
data = A*(data-Min)/(Max-Min) + B
2 Contour
data = A*(data-ContourMin)/(ContourMax-ContourMin) + B
3 Contour and Solvent

if data < ContourSolvent, data = ContourSolvent. After this, calculate the below.

data = A*(data-ContourMin)/(ContourMax-ContourMin) + B
4 No Estimation
data = A*data + B
5 Assume the density as Gaussian

data = A*Normalized(data) + B

where normalized means (average=0, SD=1)

Execution example

Input file image

Input-1VOM-N-2D.png

Min

Max
Mean
SD

SE

-18651.7 (10, 1, 0)

52942.7 (24, 39, 0)
7214.87
10067.6

125.845


Example of options only essential

Outdata-mrcImageNormalizing.png

Min

Max
Mean
SD

SE

-0.00221962 (10, 1, 0)

0.00448009 (24, 39, 0)
0.000200939
0.000942109

1.17764e-05


Option -A, -B

Case: A=1000, B=3

Outdata-m0-mrcImageNormalizing.png

Min

Max
Mean
SD

SE

0.780381 (10, 1, 0)

7.48009 (24, 39, 0)
3.20094
0.942109

0.0117764

Option -m

Case: m=5

Case: A=1, B=0
Outdata-m5-mrcImageNormalizing.png

Min

Max
Mean
SD

SE

-2.5693 (10, 1, 0)

4.5421 (24, 39, 0)
-3.70619e-12
1

0.0125

Data is Normalized as Mean=0, SD=1.

Case: A=3, B=2
Outdata1-m5-mrcImageNormalizing.png

Min

Max
Mean
SD

SE

-5.70789 (10, 1, 0)

15.6263 (24, 39, 0)
2
3

0.0375

Data is Normalized as Mean=2, SD=3.