Admin: /* Outline of the 3D reconfiguration method */

2013-06-03T08:06:20Z

‎Outline of the 3D reconfiguration method

Admin: Created page with "== Outline of the 3D reconfiguration method == There are several methods usable for 3D reconfiguration. The methods marked with Eos are the ones supported by Eos. 1. Simple B..."

2013-06-03T08:02:52Z

Created page with "== Outline of the 3D reconfiguration method == There are several methods usable for 3D reconfiguration. The methods marked with Eos are the ones supported by Eos. 1. Simple B..."

New page

== Outline of the 3D reconfiguration method ==

There are several methods usable for 3D reconfiguration. The methods marked with Eos are the ones supported by Eos.
1. Simple Backward projection (Eos): Used to perform backward projection simply.
2. Weighted backward projection (Eos): Used to give weight in the Fourier space based on the projection angle of the projected image. This method is often used when a low-pass filter is applied to one-axis projection (tomography).
3. Backward projection with a filter (not supported yet)
4. Iterative method
4.1 SIRT method (Simultaneous Iterative Reconstruction Technique)
4.2 ART method (Algebric Iterative Reconstruction Technique) (not supported yet)
5. Radon transformation method (Eos)

In the exercise, the weighted backward projection method is used.

IX-2. Practice of the 3D reconstruction
A program to perform the 3D reconfiguration method utilizing the weighted backward projection is as follows:

.3dlst.3d:
mrc2Dto3D -I $(TARGET).3dlst -o $(TARGET).3d -InterpolationMode 2 -Double -DoubleCounter $(TARGET).3dcounter -CounterThreshold 0.5 -m 1 -WeightMode 2

“$(TARGET).3dcounter” shows weight. If $(TARGET).3d and $(TARGET).3dcounter files are available, 3D images can be averaged using mrcImageAverage.

@@ Line 1: / Line 1: @@
 == Outline of the 3D reconfiguration method ==
-There are several methods usable for 3D reconfiguration. The methods marked with Eos are the ones supported by Eos.
+There are several methods usable for 3D reconfiguration. The methods to be performed by Eos are the below.
 . Simple Backward projection (Eos): Used to perform backward projection simply.
-. Weighted backward projection (Eos): Used to give weight in the Fourier space based on the projection angle of the projected image. This method is often used when a low-pass filter is applied to one-axis projection (tomography).
+. Weighted backward projection (Eos):
-. Backward projection with a filter (not supported yet)
+. Filtered Backprojection (Backward projection with a filter): Used to give weight in the Fourier space based on the projection angle of the projected image. This method is often used when a low-pass filter is applied to one-axis projection (tomography).
 . Iterative method
 .1 SIRT method (Simultaneous Iterative Reconstruction Technique)
@@ Line 13: / Line 13: @@
 IX-2.	Practice of the 3D reconstruction
   .3dlst.3d:
   	mrc2Dto3D -I $(TARGET).3dlst -o $(TARGET).3d -InterpolationMode 2 -Double -DoubleCounter $(TARGET).3dcounter -CounterThreshold 0.5 -m 1 -WeightMode 2
-“$(TARGET).3dcounter” shows weight. If $(TARGET).3d and $(TARGET).3dcounter files are available, 3D images can be averaged using mrcImageAverage.
+“$(TARGET).3dcounter” shows the weight of Fourier Transform to be filtered by back-projection. If $(TARGET).3d and $(TARGET).3dcounter files are available, 3D images can be averaged using mrcImageAverage.

3D Reconstruction: Backprojection - Revision history

Admin: /* Outline of the 3D reconfiguration method */

Admin: Created page with "== Outline of the 3D reconfiguration method == There are several methods usable for 3D reconfiguration. The methods marked with Eos are the ones supported by Eos. 1. Simple B..."