Difference between revisions of "mrcImageCTFObservation"
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====Mode: ctfMode=32==== | ====Mode: ctfMode=32==== | ||
Revision as of 02:27, 17 September 2014
mrcImageCTFObservation is Eos's Command for the simulated mrcImage in modulation when it is taken with an electron microscope.
Contents
List of option
Main option
| Option | Essential/Optional | Description | Default |
|---|---|---|---|
| -i | Essential | Input: mrcImage | NULL |
| -o | Optional | Output: mrcImage | NULL |
| -kV | Optional | Acceleration voltage[kV] | 200 |
| -Cs | Optional | Spherical aberration coefficient[mm] | 2.1 |
| -df | Optional | Defocus (under is +) [A] | 27000 |
| -A | Optional | AmplitudeContrast/PhaseContrast(Amplitude/Phase) | 0.0 |
| -Ain | Optional | IlluminationAperture[mrad] | 0.0 |
| -white | Optional | WhiteNoise(Noise/Signal) | 1.0 |
| -SinWin | Optional | RMin[A-1] RMax[A-1] | 0.1 1.0 |
| -ctfMode | Optional | ctfMode | 0 |
| -Astig | Optional | With Atigmatism: dfMin[A] dfMax[A] ASaxis[degree] | 0 0 0 |
| -c | Optional | configFile | NULL |
| -m | Optional | mode | 0 |
-ctfMode details
| Value | Description |
|---|---|
| 0 | Phase Only |
| 1 | Phase and Amplitude |
| +32 | With Illumination Angle |
| +256 | As Absolute Value |
Execution example
Input file image
 |
Min Max |
0 (0, 0, 0) 255 (49, 32, 0) |
Example of options only essential
 |
Min Max |
-73.5162 (35, 36, 0) 57.3742 (50, 50, 0) |
Option -kV
Case: kV=100
 |
Min Max |
-77.1076 (55, 33, 0) 65.1303 (29, 27, 0) |
Option -Cs
Case: Cs=2
 |
Min Max |
-73.5104 (35, 36, 0) 57.3753 (50, 50, 0) |
Option -df
Case: df=54000
 |
Min Max |
-73.2264 (52, 35, 0) 61.0619 (26, 25, 0) |
Option -Astig
Mode: Astig=(-54000, 54000, 45)
 |
Min Max |
-56.8104 (43, 35, 0) 57.875 (19, 31, 0) |
Option -ctfMode
Mode: ctfMode=1
 |
Min Max |
-73.5162 (35, 36, 0) 57.3742 (50, 50, 0) |
Mode: ctfMode=1, A=1
 |
Min Max |
-261.062 (39, 38, 0) 47.4055 (28, 25, 0) |
Mode: ctfMode=32
 |
Min Max |
-66.9938 (29, 30, 0) 96.1484 (39, 34, 0) |
Mode: ctfMode=32, Ain=0.1
 |
Min Max |
-61.8551 (28, 30, 0) 85.9497 (38, 35, 0) |
Mode: ctfMode=33, A=1, Ain=0.1
 |
Min Max |
-56.019 (27, 27, 0) 298.03 (38, 36, 0) |
Mode: ctfMode=256
 |
Min Max |
-114.328 (37, 35, 0) 68.9204 (29, 28, 0) |
Mode: ctfMode=257, A=1
 |
Min Max |
-317.886 (37, 36, 0) 73.1741 (29, 27, 0) |
Mode: ctfMode=288, Ain=0.1
 |
Min Max |
-99.5577 (37, 35, 0) 61.4533 (29, 28, 0) |
Mode: ctfMode=289, A=1, Ain=0.1
 |
Min Max |
-299.98 (37, 36, 0) 56.6101 (29, 27, 0) |
