mrcImageCTFObservation

mrcImageCTFObservation is Eos's Command that mrcImage形式の画像に電子顕微鏡で撮影した際の画像変調をシミュレートするための

List of option

Main option

Option	Essential/Optional	Description	Default
-i	Essential	Input: mrcImage	NULL
-o	Optional	Output: mrcImage	NULL
-kV	Optional	Acceleration voltage[kV]	200
-Cs	Optional	Spherical aberration coefficient[mm]	2.1
-df	Optional	Defocus (under is +) [A]	27000
-A	Optional	AmplitudeContrast/PhaseContrast(Amplitude/Phase)	0.0
-Ain	Optional	IlluminationAperture[mrad]	0.0
-white	Optional	WhiteNoise(Noise/Signal)	1.0
-SinWin	Optional	RMin[A-1] RMax[A-1]	0.1 1.0
-ctfMode	Optional	ctfMode	0
-Astig	With Atigmatism	dfMin[A] dfMax[A] ASaxis[degree]	0 0 0
-c	Optional	configFile	NULL
-m	Optional	mode	0

-ctfMode details

Value	Description
0	Phase Only
1	Phase and Amplitude
+32	With Illumination Angle
+256	As Absolute Value

Execution example

Input file image

Min Max Mean SD SE

-298.592 (37, 36, 0) 46.4642 (56, 19, 0) -21.0538 52.1224 0.65153

Example of options only essential

Min Max Mean SD SE

-66.9938 (29, 30, 0) 96.1484 (39, 34, 0) -1.94998e-08 18.286 0.228575

Option -kV

Mode: kV=100

Min Max Mean SD SE

-75.0422 (26, 28, 0) 102.125 (36, 34, 0) 2.99404e-08 20.3622 0.254527

Option -Cs

Mode: Cs=2

Min Max Mean SD SE

-67.0312 (29, 30, 0) 96.1602 (39, 34, 0) 1.87724e-08 18.2861 0.228576

Option -df

Mode: df=54000

Min Max Mean SD SE

-75.6365 (29, 26, 0) 96.8578 (54, 34, 0) -5.46698e-09 21.2739 0.265924

Option -Astig

Mode: Astig=(-54000, 54000, 45)

Min Max Mean SD SE

-62.1862 (59, 44, 0) 78.7101 (33, 53, 0) -3.56755e-08 18.5265 0.231581

Option -ctfMode

Mode: ctfMode=1

Min Max Mean SD SE

-66.9938 (29, 30, 0) 96.1484 (39, 34, 0) -1.94998e-08 18.286 0.228575

Mode: ctfMode=1, A=1

Min Max Mean SD SE

-62.9971 (27, 27, 0) 310.586 (38, 36, 0) 21.0538 58.8599 0.735749

Mode: ctfMode=32

Min Max Mean SD SE

-66.9938 (29, 30, 0) 96.1484 (39, 34, 0) -1.94998e-08 18.286 0.228575

Mode: ctfMode=32, Ain=0.1

Min Max Mean SD SE

-61.8551 (28, 30, 0) 85.9497 (38, 35, 0) 6.42321e-08 16.94 0.21175

Mode: ctfMode=33, A=1, Ain=0.1

Min Max Mean SD SE

-56.019 (27, 27, 0) 298.03 (38, 36, 0) 21.0538 57.7534 0.721917

Mode: ctfMode=256

Min Max Mean SD SE

-114.328 (37, 35, 0) 68.9204 (29, 28, 0) 1.32575e-09 18.286 0.228575

Mode: ctfMode=257, A=1

Min Max Mean SD SE

-317.886 (37, 36, 0) 73.1741 (29, 27, 0) -21.0538 58.8599 0.735749

Mode: ctfMode=288, Ain=0.1

Min Max Mean SD SE

-99.5577 (37, 35, 0) 61.4533 (29, 28, 0) -5.21633e-08 16.94 0.21175

Mode: ctfMode=289, A=1, Ain=0.1

Min Max Mean SD SE

-299.98 (37, 36, 0) 56.6101 (29, 27, 0) -21.0538 57.7534 0.721917