mrcImageAverage

mrcImageAverage is Eos's Command that creates Averaged image from some images. When dealing with a lot of Particle's images as Single particle analysis, it is effective that we get the Averaged image. Noise is reduced by an average of the image. Thus the accuracy of the Angle determined is improved, and when perfoming 3D Reconstruction, Improvement of Resolution is expected. Refer to the Averaged image for more information.

And when making the averaged image from incomplete 3-dimensional image in the direction of the projection, it is effective that we make averaged with the information such as missing area. Hence, it is effective that we use the information file of reconstructing image like DoubleCounter's file (by mrc2Dto3D setting -w) for the reconstruction. In order to also include information reflecting the number of files of the projected image, DoubleCounter's file can be used directly the average.

List of option

Option	Essential/Optional	Description	Default
-i	Essential	Input: ASCII (mrcImage file list)	NULL
-o	Essential	Output: mrcImage	NULL
-w	Optional	Input: ASCII (Weight file list): DoubleCounter's file by mrc2Dto3D	NULL
-oW	Optional	Output: mrcImage (Weight file)	NULL
-h	Optional	Help

Execution example

The average of the two-dimensional image

Input file's data

 1vom-rot0.mrc
 1vom-rot1.mrc
 1vom-rot2.mrc
 1vom-rot3.mrc

1vom-rot0.mrc details

Min Max Mean SD SE

0 73.1421 6.29881 20.4635 0.215447

1vom-rot1.mrc details

Min Max Mean SD SE

0 77.0909 6.29894 14.3204 0.223756

1vom-rot2.mrc details

Min Max Mean SD SE

0 83.9283 6.29888 15.77 0.246406

1vom-rot3.mrc details

Min Max Mean SD SE

0 87.0306 6.29884 15.437 0.241203

Output

Min Max Mean SD SE

0 70.8404 6.29887 14.3507 0.224229

The average of the three-dimensional image

Input file's data

-i 's data

1WDC-Tom-1.3d
1WDC-Tom-2.3d

1WDC-Tom-1.3d's image

xy-plane

yz-plane

Min Max Mean SD SE

-1.0037 (36, 18, 37) 1.99054 (33, 40, 32) 0.0116138 0.0958051 0.000187119

1WDC-Tom-1.3d's image

xy-plane

yz-plane

Min Max Mean SD SE

-1.085 (36, 18, 37) 2.01411 (37, 34, 35) 0.0116637 0.101949 0.000199119

-w 's data

1WDC-Tom-1.3dcounter
1WDC-Tom-2.3dcounter

1WDC-Tom-1.3dcounter

1WDC-Tom-2.3dcounter

Example of options only essential

xy-plane

yz-plane

Min Max Mean SD SE

-1.04435 (36, 18, 37) 1.74931 (33, 40, 32) 0.0116387 0.0856551 0.000167295

Option -w

Case: Setting -w's file

xy-plane

yz-plane

Min Max Mean SD SE

-1.08915 (36, 18, 37) 1.90308 (33, 40, 32) 0.0116386 0.088767 0.000173373

The weight file in the output file of -o is created when you set the -oW, can be used in the averaging it again.