mrcImageNormalizedSubtraction

mrcImageNormalizedSubtraction is Eos's Command that normalizes the density of images, and calculates the subtraction between the images. In addtion, It can calculate RMSD between images. (-rmsd: if set, file output; otherwise, standard output)

List of option

Main option

Option	Essential/Optional	Description	Default
-i1	Essential	Input1: mrcImage	NULL
-i2	Essential	Input2: mrcImage	NULL
-o	Essential	Output: mrcImage	NULL
-nori2	Optional	Output2: mrcImage	NULL
-rmsd	Optional	Output: ASCII(RMSD)	stdout (Standard output by default)
-pf	Optional	Output: ASCII(パラメータ)	stdout (Standard output by default)
-c	Optional	ConfigurationFile	NULL
-m	Optional	Mode	0
-h	Optional	Help

-m details

Value	Description
+1	Sigma!=1.0
+2	Stdout
+4	Reversed
+8	Robust Test

Execution example

Input file1 image

Min Max Mean SD SE

0 255 21.0538 47.6709 0.595886

Input file2 image

Min Max Mean SD SE

0 255 111.211 32.7239 0.409049

Example of options only essential

Output

-rmsd, -pf is standard output if not set.

a= -76.811897, b= 0.879999, r= 0.000000, 
sigma_a= 1.679392, sigma_b= 0.014486, 
RMSD: 179.359329

-o image

Min Max Mean SD SE

0 255 147.638 101.846 1.27308

-nori2 image

Min Max Mean SD SE

0 255 77.1355 90.1288 1.12661

-rmsd data

Output is written to the specified file.

RMSD: 179.359329

-pf data

Output is written to the specified file.

a= -76.811897, b= 0.879999, r= 0.000000, 
sigma_a= 1.679392, sigma_b= 0.014486, 

Option -m

m=4

-o image

Min Max Mean SD SE

0 255 102.362 100.504 1.2563